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KMID : 0606920070150040224
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Biomolecules & Therapeutics
2007 Volume.15 No. 4 p.224 ~ p.229
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Depurination of dA and dG Induced by 2-bromopropane at the Physiological Condition
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Pritam Thapa
Sherchan Jyoti
Radha Karki
Jeong Tae-Cheon
Lee Eung-Seok
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Abstract
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Depurination, the release of purine bases from nucleosides by hydrolysis of the N-glycosidic bond, gives rise to alterations of the cell genome. Although, cells have evolved mechanisms to repair these lesions, unrepaired apurinic sites have been shown to have two biological consequences: lethality and base substitution errors. 2-Bromopropane (2-BP) is used as an intermediate in the synthesis of pharmaceuticals, dyes, and other organics. In addition, 2-BP has been used as a cleaning solvent in electronics industry. But, 2-BP was found to cause reproductive and hematopoietic disorders in local workers exposed to it. We observed massive depurination after incubation of 2’-deoxyadenosine (dA) and 2’-deoxyguanosine (dG) with the excess amount 2-BP at the physiological condition (pH 7.4, 37oC), which were analyzed by HPLC and LC-MS/MS. In addition, time and dose response relationship of depurination in dA and dG induced by 2-BP at the physiological condition were investigated.
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KEYWORD
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depurination, 1-bromopropane, 2-bromopropane, time and dose response relationship
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